Dissertation Development and Application of New Methodology for 1 H - Detected MAS Solid - State NMR on Biomolecules
نویسنده
چکیده
DEUTSCH) In der hier vorgestellten Arbeit werden neuartige Festkörper-NMR (Nuclear Magnetic Resonance) Experimente vorgestellt, die eine direkte Detektion von Protonen einbeziehen. Die Technik basiert auf einer weitgehenden Verdünnung der Protonen durch Deuteronen in vollständig isotopenmarkierten, rekombinant exprimierten Proteinen und ermöglicht FestkörperNMR mit sehr schmalen Linienbreiten aller standardmäßig erfassbaren Kerne (Protonen, Stickstoff, Kohlenstoff) ohne Hochleistungsentkopplung. Zusätzlich werden Methoden für ein besonders hohes Signal-zu-Rausch durch Paramagnetic Relaxation Enhancement (PRE) entwickelt. Die so präparierten Proteine erweisen sich tauglich für eine stark verbesserte NMRCharakterisierung durch eine Vielzahl neuer Strukturund Zuordnungsexperimente, in denen Techniken aus Festkörperund Lösungs-NMR vereint werden. Dabei können hier erstmals auch Bereiche im Protein mit einbezogen werden, die langsame Dynamik vollziehen. Die Experimente finden Anwendung auf die SH3-Domäne von alpha-Spektrin, das AlzheimerPeptid Abeta1-40 und das Membranprotein Omp G. Schlagworte: Festkörper-NMR, Protonendetektion, MAS (magic angle spinning), Deuterierung
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